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Biotechnology - Proteomics: Principles and Techniques

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Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT Bombay. For more details on NPTEL visit http://nptel.iitm.ac.in

Self-Study Content
  1. Mod-01 Lec-01 Introduction to Proteomics Course

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  2. Mod-02 Lec-02 Central Dogma: Basics of DNA, RNA, Proteins

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  3. Mod-03 Lec-03 Genomics and Transcriptomics: Why proteomics?

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  4. Mod-04 Lec-04 Proteins: Amino acids and structural levels of proteins

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  5. Mod-05 Lec-05 Proteins: Folding and misfolding

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  6. Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  7. Mod-07 Lec-07 Enzymes: Basic concepts, Catalytic and Regulatory strategies

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  8. Mod-08 Lec-08 Proteomics and Systems Biology

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  9. Mod-09 Lec-09 Sample preparation for proteomics applications

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  10. Mod-10 Lec-10 Sample preparation for proteomics applications: Serum and bacterial proteome

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  11. Mod-11 Lec-11 Sample preparation for proteomics applications

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  12. Mod-12 Lec-12 Gelbased Proteomics

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  13. Mod-13 Lec-13 Gel-based Proteomics Two-dimensional electrophoresis(continued)

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  14. Mod-14 Lec-14 Gel-based Proteomics Two-dimensional electrophoresis Workflow

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  15. Mod-15 Lec-15 Two-dimensional electrophoresis: Image processing and data analysis

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  16. Mod-16 Lec-16 Two-dimensional difference gel electrophoresis (2D DIGE)

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  17. Mod-17 Lec-17 Difference gel electrophoresis (continued) Discussion and data analysis

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  18. Mod-18 Lec-18 Applications of two dimensional electrophoresis

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  19. Mod-19 Lec-19 Applications of 2-DE and DIGE

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  20. Mod-20 Lec-20 Fundamentals of mass spectrometry

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  21. Mod-21 Lec-21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF)

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  22. Mod-22 Lec-22 Liquid chromatography-Mass spectrometry (LC-MS/MS)

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  23. Mod-23 Lec-23 Hybrid-MS/MS Configurations

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  24. Mod-24 Lec-24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC)

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  25. Mod-25 Lec-25 Quantitative Proteomics: iTRAQ and TMT

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  26. Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  27. Mod-27 Lec-27 Microarray workflow: Label-based detection techniques

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  28. Mod-28 Lec-28 Microarray related concepts: Recombinational cloning Cell-free expression

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  29. Mod-29 Lec-29 Cell-free synthesis based protein microarrays

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  30. Mod-30 Lec-30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  31. Mod-31 Lec-31 Microarray work-flow: Image scanning and processing

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  32. Mod-32 Lec-32 Microarray work-flow: Data analysis

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  33. Mod-33 Lec-33 Applications of protein microarrays

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  34. Mod-34 Lec-34 Applications of cell free protein microarrays

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  35. Mod-35 Lec-35 Label-free techniques: SPR and SPRi

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  36. Mod-36 Lec-36 Label-free techniques: SPRi, Ellipsometry, Interference

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  37. Mod-37 Lec-37 Surface Plasmon Resonance: Biacore SPR and data analysis

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  38. Mod-38 Lec-38 Nanotechniques in proteomics

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  39. Mod-39 Lec-39 Detection System: Diffraction-based biosensors

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

  40. Mod-40 Lec-40 Proteomics: Advances and Challenges

    Proteomics: Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT

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